SPRYD3 Knockout cell line (Hep G2)

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SPRYD3 Knockout cell line (Hep G2) Order

SPRYD3 Knockout cell line (Hep G2)
Catalog#
YKO-HT7247
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SPRYD3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
SPRYD3 Knockout cell line (Hep G2)
Species
Human
Gene Name
SPRYD3
Gene ID
84926
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript SPRYD3-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SPRYD3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SPRYD3 gene knockout SPRYD3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SPRYD3 Knockout cell line (Hep G2)
SPRYD3 Knockout cell line (Hep G2)
Catalog#
YKO-HT7247
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

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KO Cell Lines

  • SPRYD3 Knockout cell line (U-2 OS)

    Organism: Human
    Gene Name: SPRYD3
    Gene ID: 84926
    Size: 1*10^6
    Catalog#: YKO-HT13417
    Request a Quote More details

  • SPRYD3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SPRYD3
    Gene ID: 84926
    Size: 1*10^6
    Catalog#: YKO-XN67420
    Request a Quote More details

  • SPRYD3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SPRYD3
    Gene ID: 84926
    Size: 1*10^6
    Catalog#: YKO-XN67419
    Request a Quote More details

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